Influence of ascorbic acid on chromium induced epigenetic alterations

• Uptake and translocation studies

• Gene expression analysis

• Investigation of DNA-methylation changes

Methods ​​​​​​​

• Methylation Specific PCR

• Gene expression profiling using high-throughput RT-PCR

• Quantification of metals (AAS, colorimetric assay)

• Cytotoxicity assays

• Quantification with HPLC (ascorbic acid, nucleosides)