Research focus

Epigenotoxic effects of metals and their impact on gene expression

Dermal exposition studies of metal-based nanomaterials
- Investigation and comparison of damage profiles in relevant epidermal skin cell lines
- Uptake and translocation studies

Toxicological investigations of Mineral Oil Aromatic Hydrocarbons (MOAH)
- Gene expression analysis via high-throughput RT-qPCR
- Sunlight-induced photo toxicity of PAHs and MOAH

Spectrum of methods

Methylation specific PCR
Generation of gene expression profiles by high-throughput RT-qPCR
Metal determinations via AAS and colorimetrical assays
Cytotoxicity assays

Teaching

Supervision of Master and Bachelor theses
Organization and supervision of practical laboratory courses (PCR)

Publications

Schumacher P., Fischer F., Sann J., Walter D., Hartwig A.
Impact of nano- and micro-sized Chromium(III) particles on cytotoxicity and gene expression profiles related to genomic stability in human keratinozytes and alveolar epithelial cells
Nanomaterials (2022)

Wall J., Ag Seleci D., Schworm F., Neuberger R., Link M., Hufnagel M., Schumacher P., Schulz F., Heinrich U., Wohlleben W., Hartwig A.
Comparison of Metal-Based Nanoparticles and Nanowires: Solubility, Reactivity, Bioavailability and Cellular Toxicity
Nanomaterials (2021)

Weber S., Schmidt T., Schumacher P., Kuballa T., Mildau G., Walch S. G., Hartwig A. Lachenmeier D. W.
Quantification of Mineral Oil Aromatic Hydrocarbons (MOAH) in Anhydrous Cosmetics Using ¹H NMR
Journal Of Chemistry (2019)

Oral presentations

P. Schumacher¹, F. Fischer¹, J. Sann^2,3, D. Walter^4,5, A. Hartwig¹

¹Department of Food Chemistry and Toxicology, Institute of Applied Biosciences (IAB), Karlsruhe Institute of Technology (KIT), Adenauerring 20a, 76131 Karlsruhe, Germany

²Institute of Physical Chemistry, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany

³Center for Materials Research (LaMa/ZfM), Justus-Liebig-University Giessen, Heinrich-Buff-Ring 16, 35392 Giessen, Germany

⁴Laboratories of Chemistry and Physics, Institute of Occupational and Social Medicine, Justus-Liebig-University Giessen, Aulweg 129, 35392 Giessen, Germany

⁵Institute of Inorganic and Analytical Chemistry, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany

"Impact of nano- and micro-sized Chromium(III) particles on cytotoxicity and gene expression profiles related to genomic stability in human keratinozytes and alveolar epithelial cells", 7th German Pharm-Tox Summit, 8 – 10 March 2022, Wuppertal

P. Schumacher¹, M. Kuntz¹, S. Weber², R. Paulick², G.Mildau², T. Kuballa², A. Hartwig¹

¹ Karlsruhe Institute of Technology, Karlsruhe, Germany
²CVUA Karlsruhe (Chemical and Veterinary Investigation Office), Karlsruhe, Germany

"Toxikologische Studien von MOAH aus Fraktionen kosmetisch relevanter Mineralöl-Rohstoffe", Seminar: Mineralölbestandteile in Lebensmitteln und Kosmetika, 26. - 27.03.2019, Berlin

P. Schumacher¹, M. Kuntz¹, S. Weber², R. Paulick², G.Mildau², T. Kuballa², A. Hartwig¹

¹ Karlsruhe Institute of Technology, Karlsruhe, Germany
² CVUA Karlsruhe (Chemical and Veterinary Investigation Office), Karlsruhe, Germany

"Toxikologische Studien von MOAH aus Fraktionen von Mineralöl-Rohstoffen für Kosmetika", Karlsruher Kosmetiktage 2019, Sicherheit kosmetischer Mittel, 26. - 27.03.2019, Karlsruhe

Poster Presentations

P. Schumacher¹, M. Kuntz¹, S. Weber², R. Paulick², G.Mildau², T. Kuballa², A. Hartwig¹
¹ Karlsruhe Institute of Technology, Karlsruhe, Germany
² CVUA Karlsruhe (Chemical and Veterinary Investigation Office), Karlsruhe, Germany

„Toxikologische Untersuchungen von MOAH - Update”,

Regionalverbandstagung 2019 - Lebensmittelchemische Gesellschaft, 05. - 06.03.2019, Hohenheim (DE)

P. Schumacher¹, M. Kuntz¹, S. Weber², T. Schmidt², G. Mildau², T. Kuballa², A. Hartwig¹
¹ Karlsruhe Institute of Technology, Karlsruhe, Germany
² CVUA Karlsruhe (Chemical and Veterinary Investigation Office), Karlsruhe, Germany

„Toxikologische Untersuchungen von MOAH”,

47. Deutscher Lebensmittelchemikertag, 17. - 19.09.2018, Berlin (DE)

P. Schumacher¹, R. Neuberger¹, J. Lichter², B. Blömeke², A. Hartwig¹
¹ Karlsruhe Institute of Technology, Karlsruhe, Germany
²University of Trier, Trier, Germany

„Gene expression profiling of soluble copper and copper oxide nanoparticles in skin cells”,

15^th Biennial Meeting of the DGDR - DNA Repair 2018, 09. - 12.09.2018, Karlsruhe (GER)