Research focus

Influence of ascorbic acid on chromium induced epigenetic alterations

Uptake and translocation studies
Gene expression analysis
Investigation of DNA-methylation changes

Methods

Methylation Specific PCR
Gene expression profiling using high-throughput RT-PCR
Quantification of metals (AAS, colorimetric assay)
Cytotoxicity assays
Quantification with HPLC (ascorbic acid, nucleosides)

Teaching

Supervision of master and bachelor thesis
Organization of the lab course "Biochemisches und Toxikologisches Praktikum"
Supervision of teaching lab "Toxikologisches Praktikum" (AAS)

Publications

Lumpp T., Stösser S., Fischer F., Hartwig A., Koberle B.
Role of Epigenetics for the Efficacy of Cisplatin
International Journal of Molecular Sciences (2024)

Stösser S., Lumpp T., Fischer F., Gunesch S., Schumacher P., Hartwig A.
Effect of Long-Term Low-Dose Arsenic Exposure on DNA Methylation and Gene Expression in Human Liver Cells
International Journal of Molecular Sciences (2023)

Caipa Garci A. L., Kucab J. E., Al-Serori H., Beck R. S. S., Fischer F., Hufnagel M., Hartwig A., Floeder A., Balbo S., Francies H., Garnett M., Huch M., Drost J., Zilbauer M., Arlt V. M., Phillips D. H.
Metabolic Activation of Benzo[a]pyrene by Human Tissue Organoid Cultures
International Journal of Molecular Sciences (2022)

Schumacher P., Fischer F., Sann J., Walter D., Hartwig A.
Impact of nano- and micro-sized Chromium(III) particles on cytotoxicity and gene expression profiles related to genomic stability in human keratinozytes and alveolar epithelial cells
Nanomaterials (2022)