Spectrum of methods

To assess the influence of metals and metal compounds, nanomaterials and bioactive food compounds on genomic stability in cell culture a broad range of different methods was established in our laboratory. They include:

  • the application of regular, co- and 3D-cell culture systems
    • determination of viability and cytotoxicity,
    • examination of apoptosis and cell cycle control (e.g. with flow cytometry),
    • measuring concentrations of selected metals with atom absorption spectrometry (AAS) and colorimetric methods,
    • quantitative and qualitative protein determination with Western Blot, immunofluorescence and ELISA,
  • the quantification of DNA damage and repair induction as well as mutagenicity
    • oxidative DNA damage, benzo[a]pyrene-induced DNA damage, UVC-induced DNA damage, DNA single and double strand breaks, e.g. by alkaline unwinding, micronuclei induction, HPLC with fluorescence detection,
    • examination of different DNA repair pathways (e.g. wiith reporter test systems, repair-deficient cell lines, accumulation of DNA repair proteins),
    • examination of PARP activity,
    • determination of mutagenicity by PIG-A assay,
  • the observation of selected cellular processes with live cell imaging (in a time-dependent manner),
  • the analysis of gene expression profiles with real-time RT-PCR and multiplex-PCR
    • influence on genomic stability (oxidative stress, general stress response, DNA damage response, cell cycle control, metal homeostasis, inflammatory and fibrotic responses),
  • the examination of particular and fibrous nanomaterials
    • characterization of nanomaterials,
    • air-liquid interface exposure,
    • comparison of soluble compounds with nano- and microparticles as well as fibrous materials with regard to cytotoxicity, DNA damage, gene expression profiling and other endpoints.


Schematic presentation of cell monocultures cultivated under submerged (left) or airl-liquid interface (middle) conditions and a triple culture under air-liquid interface conditions (right).
Schematic presentation of a reporterassay for identification of impaired DNA repair mechanisms.
Schematic presentation of a highthroughput RT-qPCR chip for gene expression profiling.