•  Pulmonary exposure of microscale materials

  • exposure scenarios via air-liquid interface (ALI)

  • assessment of pulmonary toxicity depending on cell culture models and materials forms

• Toxicological investigations of carbon fibres and α-quartz particles

  • investigation of cytotoxicity and genotoxicity

  • investigation of inflammatory and fibrotic potential

Methods

• Generation of gene expression profiles by high-throughput RT-qPCR

• Detection of DNA strand breaks (alkaline unwinding)

• Cytotoxicity assays

• Air-liquid interface cell culture