Investigations of epigenotoxic effects of metal compounds on genes of DNA repair considering differential methylation patterns

• Investigation of metal-induced changes in gene-specific and global DNA methylation patterns
• Gene expression analysis
• Cytotoxicity studies

Methods

• Methylation Specific PCR (MSP)
• Methylation-Sensitive High Resolution Melting (MS-HRM)
• Gene expression profiling using high-throughput RT-qPCR
• Cytotoxicity assays
• Quantification by HPLC (nucleosides)